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dna ssdna fragment  (Integrated DNA Technologies)


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    Integrated DNA Technologies dna ssdna fragment
    Dna Ssdna Fragment, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/megamer+single-stranded+dna+(ssdna)+fragments/Megamer+Single-Stranded+DNA+(ssDNA)+Fragments/pm34424588-106-18-22
    Average 92 stars, based on 34 article reviews
    dna ssdna fragment - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Generated:

    Article Title: RNA stable isotope probing and high-throughput sequencing to identify active microbial community members in a methane-driven denitrifying biofilm.
    Article Snippet: Nitrate contamination of freshwater is a global concern due to the serious health and environmental problems it causes.. According to the World Health Organisation, the maximum guideline value for drinking water is 50 mg l−1 nitrate (0.81 mmol l−1) (WHO, 2017) and the threshold for ecological change is 10.6 mg l−1 nitrate (0.17 mmol l−1) (Hickey, 2013).. Nitrate concentrations exceeding these thresholds can cause infant methaemoglobinaemia (blue baby syndrome) (Majumdar, 2003), toxic algae blooms and lake eutrophication (Morgenstern et al., 2015).

    other:

    Article Title: Easi- CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins
    Article Snippet: The ssDNA HDR donors were prepared from these cloned dsDNA templates either using the Iv TRT method as described previously [ ] or obtained from IDT (MegamerTM single-stranded Gene Fragments).

    CRISPR:

    Article Title: Osteopontin and iCD8α cells promote intestinal intraepithelial lymphocyte homeostasis
    Article Snippet: .. Briefly, intron 1/2 and intron 3/4 of the Spp-1 gene were targeted for insertion of LoxP sites by CRISPR/Cas9 ribonucleoproteins (RNP) along with a 1528 bp megamer symmetrical donor ssDNA oligonucleotide (IDT). crRNA sequences were 5’-GTGTGATAACACAGACTCAT-3’ and 5’-AACCAGTACCTTACATGTT-3’. ..

    Article Title: Osteopontin and iCD8α cells promote intestinal intraepithelial lymphocyte homeostasis
    Article Snippet: .. Briefly, intron 1/2 and intron 3/4 of the Spp-1 gene were targeted for insertion of LoxP sites by CRISPR/Cas9 ribonucleoproteins (RNP) along with a 1528 bp megamer symmetrical donor ssDNA oligonucleotide (IDT). crRNA sequences were 5’-GTGTGATAACACAGACTCAT-3’ and 5’-AACCAGTACCTTACATGTT-3’. ..

    Article Title: Girdin regulates dendrite morphogenesis and cilium position in two specialized sensory neuron types in C. elegans
    Article Snippet: .. To induce homologous recombination, an injection mixture comprised of the following reagents was injected into LP172 animals: S. pyogenes Cas9 Nuclease V3 (0.25 μg/μl, IDT), CRISPR-Cas9 tracrRNA (1 μM, IDT), unc-122p::dsRed (40 ng/μl); donor template (0.11 μg/μl, megamer ssDNA fragment, IDT) with 120-bp homology arms, crRNA (0.02μg/μl, IDT): rCrArArUrArUrUrUrCrUrGrUrCrGrUrCrArCrGrUrGrUrUrUrUrArGrArGrCrUrArUrGr CrU (r denotes RNA nucleotides). ..

    Purification:

    Article Title: The fourth annual BRDS on genome editing and silencing for precision medicines
    Article Snippet: .. Per Dr. Turk, IDT has developed a method to generate long single-stranded oligonucleotides templates up to 2 kb in length that is highly purified and sequence verified (IDT Megamers ™ single-stranded gene fragments). ..

    Sequencing:

    Article Title: The fourth annual BRDS on genome editing and silencing for precision medicines
    Article Snippet: .. Per Dr. Turk, IDT has developed a method to generate long single-stranded oligonucleotides templates up to 2 kb in length that is highly purified and sequence verified (IDT Megamers ™ single-stranded gene fragments). ..

    Homologous Recombination:

    Article Title: Girdin regulates dendrite morphogenesis and cilium position in two specialized sensory neuron types in C. elegans
    Article Snippet: .. To induce homologous recombination, an injection mixture comprised of the following reagents was injected into LP172 animals: S. pyogenes Cas9 Nuclease V3 (0.25 μg/μl, IDT), CRISPR-Cas9 tracrRNA (1 μM, IDT), unc-122p::dsRed (40 ng/μl); donor template (0.11 μg/μl, megamer ssDNA fragment, IDT) with 120-bp homology arms, crRNA (0.02μg/μl, IDT): rCrArArUrArUrUrUrCrUrGrUrCrGrUrCrArCrGrUrGrUrUrUrUrArGrArGrCrUrArUrGr CrU (r denotes RNA nucleotides). ..

    Injection:

    Article Title: Girdin regulates dendrite morphogenesis and cilium position in two specialized sensory neuron types in C. elegans
    Article Snippet: .. To induce homologous recombination, an injection mixture comprised of the following reagents was injected into LP172 animals: S. pyogenes Cas9 Nuclease V3 (0.25 μg/μl, IDT), CRISPR-Cas9 tracrRNA (1 μM, IDT), unc-122p::dsRed (40 ng/μl); donor template (0.11 μg/μl, megamer ssDNA fragment, IDT) with 120-bp homology arms, crRNA (0.02μg/μl, IDT): rCrArArUrArUrUrUrCrUrGrUrCrGrUrCrArCrGrUrGrUrUrUrUrArGrArGrCrUrArUrGr CrU (r denotes RNA nucleotides). ..



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    The site-specific insertion of linear <t>DNA</t> fragments into the dsx gene of the honeybee. a) Schematic presentation of the DNA fragments employed. b) Scheme of the CRISPR/Cas9-mediated HDR. The blue box indicates the new <t>DNA</t> <t>fragment</t> that needs to be integrated. The black boxes indicate the homologous arms to the left and right. Gray boxes show the remaining part of the exon. Arrows above a box indicate the position of the oligonucleotide primers for amplifications. c) Amplicons from different individuals were analyzed by size in 1% agarose gel. Black and white reversed pictures of ethidium bromide stained gels are shown. d) The expected nucleotide sequence after locus-specific insertions. wt sequences of dsx gene above and the expected sequence after insertion for comparison with (e). e) The detected nucleotide sequences at the target site of our homozygous mutated individuals. wt, wildtype individual (noninjected).
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    Application of the entropy-based detection threshold. The relationships between expected and predicted concentrations of the standards (A) before and (B) after the detection threshold was applied and standards that failed to meet the detection threshold were removed. The predicted gene copies per µL of DNA extract were determined with equation S1 for all the standards across all samples, including the 20% and 1% downsampled results (results of replicate downsampled sets are reported as single values with standard deviations). The black line represents the linear regressions; green and orange points represent values of the dsDNA and ssDNA standards, respectively.

    Journal: bioRxiv

    Article Title: Evaluating limitations of quantitative metagenomics with synthetic dsDNA and ssDNA standards

    doi: 10.1101/2022.07.08.499345

    Figure Lengend Snippet: Application of the entropy-based detection threshold. The relationships between expected and predicted concentrations of the standards (A) before and (B) after the detection threshold was applied and standards that failed to meet the detection threshold were removed. The predicted gene copies per µL of DNA extract were determined with equation S1 for all the standards across all samples, including the 20% and 1% downsampled results (results of replicate downsampled sets are reported as single values with standard deviations). The black line represents the linear regressions; green and orange points represent values of the dsDNA and ssDNA standards, respectively.

    Article Snippet: A random selection of five sequences from the potential candidates were selected with GC contents of 31.7, 40, 45, 50, and 60% and made into Megamer® ssDNA fragments without the complementary strand (IDT, Coralville, IA, Table S8).

    Techniques:

    The site-specific insertion of linear DNA fragments into the dsx gene of the honeybee. a) Schematic presentation of the DNA fragments employed. b) Scheme of the CRISPR/Cas9-mediated HDR. The blue box indicates the new DNA fragment that needs to be integrated. The black boxes indicate the homologous arms to the left and right. Gray boxes show the remaining part of the exon. Arrows above a box indicate the position of the oligonucleotide primers for amplifications. c) Amplicons from different individuals were analyzed by size in 1% agarose gel. Black and white reversed pictures of ethidium bromide stained gels are shown. d) The expected nucleotide sequence after locus-specific insertions. wt sequences of dsx gene above and the expected sequence after insertion for comparison with (e). e) The detected nucleotide sequences at the target site of our homozygous mutated individuals. wt, wildtype individual (noninjected).

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Highly efficient site-specific integration of DNA fragments into the honeybee genome using CRISPR/Cas9

    doi: 10.1093/g3journal/jkac098

    Figure Lengend Snippet: The site-specific insertion of linear DNA fragments into the dsx gene of the honeybee. a) Schematic presentation of the DNA fragments employed. b) Scheme of the CRISPR/Cas9-mediated HDR. The blue box indicates the new DNA fragment that needs to be integrated. The black boxes indicate the homologous arms to the left and right. Gray boxes show the remaining part of the exon. Arrows above a box indicate the position of the oligonucleotide primers for amplifications. c) Amplicons from different individuals were analyzed by size in 1% agarose gel. Black and white reversed pictures of ethidium bromide stained gels are shown. d) The expected nucleotide sequence after locus-specific insertions. wt sequences of dsx gene above and the expected sequence after insertion for comparison with (e). e) The detected nucleotide sequences at the target site of our homozygous mutated individuals. wt, wildtype individual (noninjected).

    Article Snippet: The 794-bp long Myc + HA DNA fragment was synthesized as a single-stranded DNA fragment (IDT Integrated DNA Technologies, Coralville, IA: Megamer Single-Stranded DNA Fragments).

    Techniques: CRISPR, Agarose Gel Electrophoresis, Staining, Sequencing, Comparison

    The DNA fragment insertions into the dsx gene.

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Highly efficient site-specific integration of DNA fragments into the honeybee genome using CRISPR/Cas9

    doi: 10.1093/g3journal/jkac098

    Figure Lengend Snippet: The DNA fragment insertions into the dsx gene.

    Article Snippet: The 794-bp long Myc + HA DNA fragment was synthesized as a single-stranded DNA fragment (IDT Integrated DNA Technologies, Coralville, IA: Megamer Single-Stranded DNA Fragments).

    Techniques: